Journal: Cancer Science

Article Title: Adjuvant for vaccine immunotherapy of cancer – focusing on Toll‐like receptor 2 and 3 agonists for safely enhancing antitumor immunity

doi: 10.1111/cas.12824

Figure Lengend Snippet: Adjuvants in development for human vaccines

Article Snippet: Poly(I:C)LC (TLR3) , n/a , Cancer (IMOxine, phase I, Hybridon Inc.).

Techniques: Formulation, Clinical Proteomics, Emulsion

Journal: Journal of Veterinary Science

Article Title: Toll-like receptor 3-stimulation and aggregate-formation synergistically enhances anti-inflammatory activity of feline mesenchymal stem cells

doi: 10.4142/jvs.23330

Figure Lengend Snippet: IDO-1, indoleamine 2,3-dioxygenase-1; bFGF, basic fibroblast growth factor; TLR3, toll-like receptor 3; fAdMSC, feline adipose-derived mesenchymal stem cell. * p < 0.05.

Article Snippet: TLR3 was stimulated by suspending fAdMSCs were suspended in α-MEM (Nacalai Tesque, Japan) containing 15% fetal bovine serum (HyClone, GE Healthcare UK Ltd., England), penicillin and streptomycin (100 units/mL and 100 μg/mL, respectively, FUJIFILM Wako Pure Chemical Corporation, Japan) (hereafter, culture medium), seeding in wells of a six-well plate (AGC Techno Glass, Japan), and incubating them with a TLR3 ligand (poly [I:C], 1 μg/mL, FUJIFILM Wako Pure Chemical Corporation) for 1 h at 37°C in 5% CO 2 and 95% air.

Techniques: Derivative Assay

Journal: Journal of Veterinary Science

Article Title: Toll-like receptor 3-stimulation and aggregate-formation synergistically enhances anti-inflammatory activity of feline mesenchymal stem cells

doi: 10.4142/jvs.23330

Figure Lengend Snippet: IDO-1, indoleamine 2,3-dioxygenase-1; TLR3, toll-like receptor 3; fAdMSC, feline adipose-derived mesenchymal stem cell.

Article Snippet: TLR3 was stimulated by suspending fAdMSCs were suspended in α-MEM (Nacalai Tesque, Japan) containing 15% fetal bovine serum (HyClone, GE Healthcare UK Ltd., England), penicillin and streptomycin (100 units/mL and 100 μg/mL, respectively, FUJIFILM Wako Pure Chemical Corporation, Japan) (hereafter, culture medium), seeding in wells of a six-well plate (AGC Techno Glass, Japan), and incubating them with a TLR3 ligand (poly [I:C], 1 μg/mL, FUJIFILM Wako Pure Chemical Corporation) for 1 h at 37°C in 5% CO 2 and 95% air.

Techniques: Derivative Assay

Journal: Journal of Veterinary Science

Article Title: Toll-like receptor 3-stimulation and aggregate-formation synergistically enhances anti-inflammatory activity of feline mesenchymal stem cells

doi: 10.4142/jvs.23330

Figure Lengend Snippet: IDO-1, indoleamine 2,3-dioxygenase-1; bFGF, basic fibroblast growth factor; TLR3, toll-like receptor 3; fAdMSC, feline adipose-derived mesenchymal stem cell. * p < 0.05.

Article Snippet: TLR3 was stimulated by suspending fAdMSCs were suspended in α-MEM (Nacalai Tesque, Japan) containing 15% fetal bovine serum (HyClone, GE Healthcare UK Ltd., England), penicillin and streptomycin (100 units/mL and 100 μg/mL, respectively, FUJIFILM Wako Pure Chemical Corporation, Japan) (hereafter, culture medium), seeding in wells of a six-well plate (AGC Techno Glass, Japan), and incubating them with a TLR3 ligand (poly [I:C], 1 μg/mL, FUJIFILM Wako Pure Chemical Corporation) for 1 h at 37°C in 5% CO 2 and 95% air.

Techniques: Derivative Assay

Journal: Journal of Veterinary Science

Article Title: Toll-like receptor 3-stimulation and aggregate-formation synergistically enhances anti-inflammatory activity of feline mesenchymal stem cells

doi: 10.4142/jvs.23330

Figure Lengend Snippet: IDO-1, indoleamine 2,3-dioxygenase-1; bFGF, basic fibroblast growth factor; COX-2, cyclooxygenase-2; TNF-α, tumor necrosis factor-α; LPS, lipopolysaccharide; TLR3, toll-like receptor 3; fAdMSC, feline adipose-derived mesenchymal stem cell. * p < 0.05.

Article Snippet: TLR3 was stimulated by suspending fAdMSCs were suspended in α-MEM (Nacalai Tesque, Japan) containing 15% fetal bovine serum (HyClone, GE Healthcare UK Ltd., England), penicillin and streptomycin (100 units/mL and 100 μg/mL, respectively, FUJIFILM Wako Pure Chemical Corporation, Japan) (hereafter, culture medium), seeding in wells of a six-well plate (AGC Techno Glass, Japan), and incubating them with a TLR3 ligand (poly [I:C], 1 μg/mL, FUJIFILM Wako Pure Chemical Corporation) for 1 h at 37°C in 5% CO 2 and 95% air.

Techniques: Derivative Assay

Journal: Molecular Vision

Article Title: Increase in peripheral blood mononuclear cell Toll-like receptor 2/3 expression and reactivity to their ligands in a cohort of patients with wet age-related macular degeneration

doi:

Figure Lengend Snippet: List of all primer sequences.

Article Snippet: PBMC cytokine production in response to the TLR2 ligand (PGN, 5 μg/ml; Fluka, Buchs, Switzerland) or the TLR3 ligand (poly(I:C), 50 μg/ml; Fluka) was measured at 48 h after ligand stimulation.

Techniques:

Journal: Molecular Vision

Article Title: Increase in peripheral blood mononuclear cell Toll-like receptor 2/3 expression and reactivity to their ligands in a cohort of patients with wet age-related macular degeneration

doi:

Figure Lengend Snippet: Expressions of toll-like receptor 1 (TLR1)-10 mRNA in peripheral blood mononuclear cells (PBMCs) of wet age-related macular degeneration (AMD) patients. TLR2 and TLR3 mRNA expressions were higher in the wet AMD group (n=15) than in the control group (n=15; t-test, * p<0.05).

Article Snippet: PBMC cytokine production in response to the TLR2 ligand (PGN, 5 μg/ml; Fluka, Buchs, Switzerland) or the TLR3 ligand (poly(I:C), 50 μg/ml; Fluka) was measured at 48 h after ligand stimulation.

Techniques: Control

Journal: Molecular Vision

Article Title: Increase in peripheral blood mononuclear cell Toll-like receptor 2/3 expression and reactivity to their ligands in a cohort of patients with wet age-related macular degeneration

doi:

Figure Lengend Snippet: Toll-like receptor 2 (TLR2) and TLR3 protein expressions in peripheral blood mononuclear cells (PBMCs) of wet age-related macular degeneration (AMD) patients compared to healthy control. Representative TLR2 and TLR3 protein expressions of PBMC from patients (n=6; A , C ) and healthy controls (n=6; B , D ) measured with flow cytometer (FCM). Expressions of TLR2, TLR3 protein were higher in PBMCs of AMD group than in the control group ( E , F ). Mann-Whitney test, **p<0.01.

Article Snippet: PBMC cytokine production in response to the TLR2 ligand (PGN, 5 μg/ml; Fluka, Buchs, Switzerland) or the TLR3 ligand (poly(I:C), 50 μg/ml; Fluka) was measured at 48 h after ligand stimulation.

Techniques: Control, Flow Cytometry, MANN-WHITNEY

Journal: Molecular Vision

Article Title: Increase in peripheral blood mononuclear cell Toll-like receptor 2/3 expression and reactivity to their ligands in a cohort of patients with wet age-related macular degeneration

doi:

Figure Lengend Snippet: Increasing of peripheral blood mononuclear cell (PBMC) toll-like receptor 2 (TLR2) and TLR3 expressions and their reactivity to TLR ligands in wet age-related macular degeneration (AMD) patients. PBMCs were stimulated with PGN (5 μg/ml) and PolyI;C (50 μg/ml) respectively for 48 h. The PBMCs of the wet AMD patients (n=11) produced much higher IL-6 and IL-8 than the controls (n=11) in response to PGN, and much higher IL-6 than the controls in response to PolyI:C ( A , B ). The productions of MCP-1and VEGF showed no significant difference between the two groups ( C , D ) Mann–Whitney test, *p<0.05, **p<0.01.

Article Snippet: PBMC cytokine production in response to the TLR2 ligand (PGN, 5 μg/ml; Fluka, Buchs, Switzerland) or the TLR3 ligand (poly(I:C), 50 μg/ml; Fluka) was measured at 48 h after ligand stimulation.

Techniques: Produced, MANN-WHITNEY